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GlycoFlux™ MMLV-Human MGAT5 Viral Particle

CAT#: GLJF-1025-JF342

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SPECIFIC INQUIRY
Antibiotic Resistance:
Fluorescent Reporters:
Titer:
Size:
Specifications Quality Control Target Information Related Products
Specifications
Description
GlycoFlux™ MMLV-MGAT5 Viral Particle encodes MGAT5, catalyzing β1,6-GlcNAc addition to bi-antennary N-glycans to modulate cell-cell interactions; the vector establishes stable mammalian expression lines for galectin-lattice biology and migration/adhesion studies.
Product Type
Recombinant MMLV Retrovirus
Target
MGAT5
Species
Human
Promotor
MMLV 5' LTR promoter
Packaging System
Retrovirus
Packaging Cell
HEK293T
Application
This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.
Shipping
Shipped on dry ice with temperature held at or below -80°C.
Storage
Store at -80°C to maintain their titer and stability.
Handling Notes
Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer
These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Quality Control
Titer Assay
qPCR
Mycoplasma Test
Negative
Sterility
Sterility testing confirmed no microbial contamination.
Transduction Evaluation
On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation
Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.
Target Information
Full Name
Alpha-1,6-mannosylglycoprotein 6-Beta-O-acetylglucosaminyltransferase
Alternative Name
GNT-V; MGAT5A
Location
2q21.2-q21.3
Gene ID
4249
Summary
Catalyzes the beta-1,6 addition of GlcNAc to biantennary N-glycans, affecting cell-cell interactions.
For Research Use Only.
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