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GlycoFlux™ MSCV-Human GCK Viral Particle (CAT#: GLJF-1025-JF682)

Datasheet

SPECIFIC INQUIRY
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Description GlycoFlux™ MSCV-GCK Viral Particle establishes long-term glucokinase expression by MSCV integration, enhancing glucose sensing for insulin secretion and hepatic glucose uptake.
Product Type Recombinant MSCV Retrovirus
Target GCK
Species Human
Promotor MSCV 5' LTR promoter
Packaging System Retrovirus
Packaging Cell HEK293T
Application This MSCV vector is a common retroviral vector system used to achieve high-efficiency, stable gene delivery into target cells, such as hematopoietic stem cells. It ensures long-term, sustained expression by stably integrating the gene into the host cell's genome.
Shipping Dispatched on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to preserve titer and stability.
Handling Notes Store at -80°C and avoid repeated freeze-thaw cycles to preserve viral titer. For safety, handle materials in a biological safety cabinet while wearing appropriate PPE (lab coat, gloves, and eye protection).
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation Creative Biolabs offers, upon request, in vitro and in vivo transduction assays to verify lentiviral targeting and to quantify transgene expression and functional output.
Insert Identity Confirmation All Creative Biolabs retroviral vectors undergo PCR-based verification of proviral integration. Cells are transduced across a dilution series of the retroviral preparation; after several days, genomic DNA is isolated, and a defined region of the expected insert is PCR-amplified to confirm correct identity and integration.
Full Name Glucokinase
Alternative Name GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; PNDM1; FGQTL3
Location 7p13
Gene ID 2645
Summary This gene encodes a hexokinase isoform that phosphorylates glucose to glucose-6-phosphate without feedback inhibition, remaining active during high glucose. It is important for glucose-stimulated insulin secretion in the pancreas and glucose storage in the liver. Mutations are linked to diabetes and hypoglycemia.
For Research Use Only.
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