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| Description | GlycoFlux™ MSCV-PPP1R42 Viral Particle delivers PPP1R42 with MSCV to stably integrate and sustain expression in HSCs and lymphoid progenitors, allowing long-term quantification of centrosome integrity, spindle organization, and PP1-γ-tubulin coordination under a non-replicating safety design. |
| Product Type | Recombinant MSCV Retrovirus |
| Target | PPP1R42 |
| Species | Human |
| Promotor | MSCV 5' LTR promoter |
| Packaging System | Retrovirus |
| Packaging Cell | HEK293T |
| Application | This MSCV vector is a common retroviral vector system used to achieve high-efficiency, stable gene delivery into target cells, such as hematopoietic stem cells. It ensures long-term, sustained expression by stably integrating the gene into the host cell's genome. |
| Shipping | Dispatched on dry ice with temperature held at or below -80°C. |
| Storage | Store at -80°C to preserve titer and stability. |
| Handling Notes | Store at -80°C and avoid repeated freeze-thaw cycles to preserve viral titer. For safety, handle materials in a biological safety cabinet while wearing appropriate PPE (lab coat, gloves, and eye protection). |
| Product Disclaimer | These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application. |
| Titer Assay | qPCR |
| Mycoplasma Test | Negative |
| Sterility | Sterility testing confirmed no microbial contamination. |
| Transduction Evaluation | Creative Biolabs offers, upon request, in vitro and in vivo transduction assays to verify lentiviral targeting and to quantify transgene expression and functional output. |
| Insert Identity Confirmation | All Creative Biolabs retroviral vectors undergo PCR-based verification of proviral integration. Cells are transduced across a dilution series of the retroviral preparation; after several days, genomic DNA is isolated, and a defined region of the expected insert is PCR-amplified to confirm correct identity and integration. |
| Full Name | Protein Phosphatase 1 Regulatory Subunit 42 |
| Alternative Name | dtr; TLLR; TLRR; LRRC67 |
| Location | 8q13.1 |
| Gene ID | 286187 |
| Summary | This protein binds gamma-tubulin and PP1, serving as a positive regulator of PP1 to preserve centrosome organization. |