Description

GlycoFlux™ MSCV-SIGLEC12 Viral Particle adopts Murine Stem Cell Virus (MSCV) delivery for SIGLEC12, a CD33-related receptor with ITIM and SLAM-like motifs that recruit SHP1/2; the replication-incompetent, genome-integrating vector enables sustained macrophage-inhibitory signaling models.

Product Type

Recombinant MSCV Retrovirus

Target

SIGLEC12

Species

Human

Promotor

MSCV 5' LTR promoter

Packaging System

Retrovirus

Packaging Cell

HEK293T

Application

This MSCV vector is a common retroviral vector system used to achieve high-efficiency, stable gene delivery into target cells, such as hematopoietic stem cells. It ensures long-term, sustained expression by stably integrating the gene into the host cell's genome.

Shipping

Dispatched on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to preserve titer and stability.

Handling Notes

Store at -80°C and avoid repeated freeze-thaw cycles to preserve viral titer. For safety, handle materials in a biological safety cabinet while wearing appropriate PPE (lab coat, gloves, and eye protection).

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Titer Assay	qPCR
Mycoplasma Test	Negative
Sterility	Sterility testing confirmed no microbial contamination.
Transduction Evaluation	Creative Biolabs offers, upon request, in vitro and in vivo transduction assays to verify lentiviral targeting and to quantify transgene expression and functional output.
Insert Identity Confirmation	All Creative Biolabs retroviral vectors undergo PCR-based verification of proviral integration. Cells are transduced across a dilution series of the retroviral preparation; after several days, genomic DNA is isolated, and a defined region of the expected insert is PCR-amplified to confirm correct identity and integration.

Full Name	Sialic Acid Binding Ig Like Lectin 12
Alternative Name	S2V; SLG; SIGLECL1; Siglec-XII; Sialic Acid Binding Ig Like Lectin
Location	19q13.41
Gene ID	89858
Summary	This gene encodes a member of the SIGLEC3-like subfamily, characterized by extracellular V-set and C2-set immunoglobulin-like domains, and cytoplasmic motifs ITIM and SLAM-like. Tyrosine phosphorylation of the protein recruits SHP1 and SHP2, functioning as an inhibitory receptor in macrophage signaling regulation. The gene is located on 19q13.4 and undergoes alternative splicing to produce multiple transcript variants.

GlycoFlux™ MSCV-Human SIGLEC12 Viral Particle (CAT#: GLJF-1025-JF614)

Specifications

Quality Control

Target Information

Related products