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Quantitative Human ACP3 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX252)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human ACP3 (glycosylated) sandwich ELISA kit is designed to detect human acid phosphatase 3 (ACP3) levels. ACP3 is an enzyme that is found in the acrosome of sperm. This protein plays a role in fertilization, which is involved in the breakdown of the zona pellucida. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.091 ng/mL, which can accurately detect low concentrations of ACP3 in the sample.
Target ACP3
N-Glycosylation Site 94, 220, 333
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.091 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Acid phosphatase 3
Alternate Names ACP3; Acid phosphatase 3; ACPP; PAP; Prostatic specific acid phosphatase
Uniprot No. P15309
Application The quantitative human ACP3 (glycosylated) sandwich ELISA kit allows researchers to determine ACP3 levels in biological samples. It can be used in studies examining male fertility, sperm functions, and fertilization processes. The kit is applicable in reproductive biology research and the development of new fertility treatments.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-105%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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