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Quantitative Human ADRβ1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX204

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Specifications
Product Description
The quantitative human ADRβ1 (glycosylated) sandwich ELISA kit is designed to detect human adrenergic receptor β1 (ADRβ1) levels. ADRβ1 is a G protein-coupled receptor primarily found in the heart, mediating the effects of catecholamines like epinephrine and norepinephrine, and playing a crucial role in regulating heart rate and contractility. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.163 ng/mL, which can accurately detect low concentrations of ADRβ1 in the sample.
Target
ADRβ1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.163 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1.75 ng/mL-18 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Adrenergic receptor β1
Alternate Names
ADRβ1; Adrenergic receptor β1; ADRB1R; B1AR; β1 Adrenoceptor
Uniprot No.
N-Glycosylation Site
15
Application Notes
Application
The quantitative human ADRβ1 (glycosylated) sandwich ELISA kit is valuable in research investigating cardiovascular diseases, particularly heart failure and arrhythmias, where ADRβ1 expression and function are critical. Researchers utilize this ELISA to quantify ADRβ1 levels, providing insights into receptor regulation and potential therapeutic applications.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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