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Quantitative Human AMIGO1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX671

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Specifications
Product Description
The quantitative human AMIGO1 (glycosylated) sandwich ELISA kit is designed to detect human adhesion molecule with Ig like domain protein 1 (AMIGO1) levels. AMIGO1 is a transmembrane protein that belongs to the immunoglobulin superfamily, which plays a role in cell adhesion and cell signaling. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.103 ng/mL, which can accurately detect low concentrations of AMIGO1 in the sample.
Target
AMIGO1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.103 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Adhesion molecule with Ig like domain protein 1
Alternate Names
AMIGO1; Adhesion molecule with Ig like domain protein 1; ALI2; Alivin-2; Amphoterin-induced protein 1
Uniprot No.
N-Glycosylation Site
72, 264, 315, 349, 360
Application Notes
Application
The quantitative human AMIGO1 (glycosylated) sandwich ELISA kit is used to assess AMIGO1 levels in biological samples. It is applicable in studies of neuroscience, cell adhesion, and neuronal development. The kit is valuable in fields such as neurobiology and cell biology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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