Quantitative Human CD83 (Glycosylated) Sandwich ELISA Kit

The quantitative human CD83 (glycosylated) sandwich ELISA kit is designed to detect human cluster of differentiation 83 (CD83) levels. CD83 is a transmembrane glycoprotein that is primarily expressed on mature dendritic cells (DCs). This protein is considered a marker of mature DCs and is essential for their ability to present antigens to T cells and trigger an adaptive immune response. The kit is suitable for various biological samples such as cell lysates, serum, plasma. Its sensitivity is 20.026 pg/mL, which can accurately detect low concentrations of CD83 in the sample.

CD83

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Cell lysates, serum, plasma

100 μL

20.026 pg/mL

Quantitative sandwich ELISA

100 pg/mL-4000 pg/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Cluster of differentiation 83

CD83; Cluster of differentiation 83; HB15; B-cell activation protein; Cell surface protein HB15

79, 96, 117

The quantitative human CD83 (glycosylated) sandwich ELISA kit is a valuable tool for immunology research, particularly in studies of dendritic cell biology and immune regulation. Researchers utilize it to measure CD83 levels in various experimental systems. The kit has applications in studies of immune responses to infection, cancer, and transplantation, as well as in the development of immunotherapies.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-90%;
Plasma sample: n=4, 80-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.