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Quantitative Human CD83 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX509)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human CD83 (glycosylated) sandwich ELISA kit is designed to detect human cluster of differentiation 83 (CD83) levels. CD83 is a transmembrane glycoprotein that is primarily expressed on mature dendritic cells (DCs). This protein is considered a marker of mature DCs and is essential for their ability to present antigens to T cells and trigger an adaptive immune response. The kit is suitable for various biological samples such as cell lysates, serum, plasma. Its sensitivity is 20.026 pg/mL, which can accurately detect low concentrations of CD83 in the sample.
Target CD83
N-Glycosylation Site 79, 96, 117
Sample Types Cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 20.026 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 100 pg/mL-4000 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Cluster of differentiation 83
Alternate Names CD83; Cluster of differentiation 83; HB15; B-cell activation protein; Cell surface protein HB15
Uniprot No. Q01151
Application The quantitative human CD83 (glycosylated) sandwich ELISA kit is a valuable tool for immunology research, particularly in studies of dendritic cell biology and immune regulation. Researchers utilize it to measure CD83 levels in various experimental systems. The kit has applications in studies of immune responses to infection, cancer, and transplantation, as well as in the development of immunotherapies.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-90%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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