Quantitative Human DSPP (Glycosylated) Sandwich ELISA Kit

The quantitative human DSPP (glycosylated) sandwich ELISA kit is designed to detect human dentin sialophosphoprotein (DSPP) levels. DSPP is a precursor protein that is processed into dentin sialoprotein (DSP) and dentin phosphoprotein (DPP). DSPP is predominantly expressed in odontoblasts. It plays a crucial role in dentin formation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.681 ng/mL, which can accurately detect low concentrations of DSPP in the sample.

DSPP

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.681 ng/mL

Quantitative sandwich ELISA

1.75 ng/mL-85 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Dentin sialophosphoprotein

DSPP; Dentin sialophosphoprotein; DSP; DFNA39; DPP; Dentin phosphoprotein

41, 49, 81, 130, 150, 190, 191, 209, 222, 275, 336, 387

The quantitative human DSPP (glycosylated) sandwich ELISA kit is used to measure DSPP levels in biological samples. This kit is applicable in studies examining dentin formation and tooth development. It is also relevant to studies investigating the role of DSPP in the biomineralization process and its involvement in dental disorders.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.