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Quantitative Human EZH2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX610

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Specifications
Product Description
The quantitative human EZH2 (glycosylated) sandwich ELISA kit is designed to detect human enhancer of zeste homolog 2 (EZH2) levels. EZH2 is a key component of the Polycomb repressive complex 2 (PRC2), which plays a role in gene silencing. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.098 ng/mL, which can accurately detect low concentrations of EZH2 in the sample.
Target
EZH2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.098 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Enhancer of zeste homolog 2
Alternate Names
EZH2; Enhancer of zeste homolog 2; KMT6A; Lysine N-methyltransferase 6
Uniprot No.
O-Glycosylation Site
75
Application Notes
Application
The quantitative human EZH2 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated EZH2 concentration in biological samples. The kit finds application in research related to epigenetics, gene regulation, and cancer biology. It is valuable in studies investigating the role of histone methylation in cellular processes.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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