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Quantitative Human HGFAC (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX524

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Specifications
Product Description
The quantitative human HGFAC (glycosylated) sandwich ELISA kit is designed to detect human hepatocyte growth factor activator (HGFAC) levels. HGFAC is a serine protease, which activates hepatocyte growth factor (HGF) by proteolytic cleavage. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 7.034 pg/mL, which can accurately detect low concentrations of HGFAC in the sample.
Target
HGFAC
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
7.034 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
20 pg/mL-800 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Hepatocyte growth factor activator
Alternate Names
HGFAC; Hepatocyte growth factor activator; HGFA; HGFAP
Uniprot No.
N-Glycosylation Site
48, 290, 468, 546
Application Notes
Application
The quantitative human HGFAC (glycosylated) sandwich ELISA kit is a valuable tool for research in cell biology and tissue regeneration, particularly in studies of growth factor activation. Researchers use it to measure HGFAC levels in biological fluids and tissue extracts. The kit has applications in studies of liver diseases, cancer, and wound healing.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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