Quantitative Human HIF1α (Glycosylated) Sandwich ELISA Kit

The quantitative human HIF1α (glycosylated) sandwich ELISA kit is designed to detect human hypoxia inducible factor 1α (HIF1α) levels. HIF1α is a transcription factor that plays a crucial role in cellular responses to low oxygen levels (hypoxia). The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.099 ng/mL, which can accurately detect low concentrations of HIF1α in the sample.

HIF1α

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

0.099 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Hypoxia inducible factor 1α

HIF1α; Hypoxia inducible factor 1α; HIF1-A; MOP1; ARNT-interacting protein

18

The quantitative human HIF1α (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated HIF1α levels in biological samples. The kit is applicable in studies of hypoxia, angiogenesis, and tumor biology. It is useful in fields such as oncology and physiology.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.