Quantitative Human HIF1α (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX617)

Name: Quantitative Human HIF1α (Glycosylated) Sandwich ELISA Kit
SKU: GLJF-0525-HX617
Rating: 5 (1 reviews)

Datasheet

Size	Qty	Add To Basket
1×48 T
1×96 T

Product Description	The quantitative human HIF1α (glycosylated) sandwich ELISA kit is designed to detect human hypoxia inducible factor 1α (HIF1α) levels. HIF1α is a transcription factor that plays a crucial role in cellular responses to low oxygen levels (hypoxia). The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.099 ng/mL, which can accurately detect low concentrations of HIF1α in the sample.
Target	HIF1α
N-Glycosylation Site	18
Sample Types	Tissue homogenates, cell lysates, serum, plasma
Sample Volume	100 μL
Sensitivity	0.099 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	1 ng/mL-10 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Hypoxia inducible factor 1α
Alternate Names	HIF1α; Hypoxia inducible factor 1α; HIF1-A; MOP1; ARNT-interacting protein
Uniprot No.	Q16665

Application	The quantitative human HIF1α (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated HIF1α levels in biological samples. The kit is applicable in studies of hypoxia, angiogenesis, and tumor biology. It is useful in fields such as oncology and physiology.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 80-95%; Plasma sample: n=4, 85-100%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.

For Research Use Only.

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