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Quantitative Human HS6ST2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX744

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Specifications
Product Description
The quantitative human HS6ST2 (glycosylated) sandwich ELISA kit is designed to detect human heparan sulfate-6-O-sulfotransferase 2 (HS6ST2) levels. HS6ST2 is an enzyme that is involved in the synthesis of heparan sulfate. Heparan sulfate is a complex carbohydrate. It plays a role in various biological processes. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.074 ng/mL, which can accurately detect low concentrations of HS6ST2 in the sample.
Target
HS6ST2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.074 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Heparan sulfate-6-O-sulfotransferase 2
Alternate Names
HS6ST2; Heparan sulfate-6-O-sulfotransferase 2; H6ST2_HUMAN; HS6ST-2
Uniprot No.
N-Glycosylation Site
209, 404, 460, 544, 556, 564, 589, 592
Application Notes
Application
The quantitative human HS6ST2 (glycosylated) sandwich ELISA kit is useful for measuring HS6ST2 levels in the samples. This measurement is relevant to research on carbohydrate synthesis and enzyme functions. The kit also has potential applications in investigations of cell signaling pathways.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 95-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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