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Quantitative Human HSP90AB1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX200

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Specifications
Product Description
The quantitative human HSP90AB1 (glycosylated) sandwich ELISA kit is designed to detect human heat shock protein 90kda αB1 (HSP90AB1) levels. HSP90AB1 is a molecular chaperone that plays a critical role in protein folding, stabilization, and signal transduction pathways, influencing cellular responses to stress. Its activity is implicated in various diseases, including cancer and neurodegenerative disorders. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.731 ng/mL, which can accurately detect low concentrations of HSP90AB1 in the sample.
Target
HSP90AB1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.731 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
2.50 ng/mL-100 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Heat shock protein 90kda αB1
Alternate Names
HSP90AB1; Heat shock protein 90kda αB1; HSP90B; HSPCB
Uniprot No.
O-Glycosylation Site
434, 452
Application Notes
Application
The quantitative human HSP90AB1 (glycosylated) sandwich ELISA kit is valuable in research investigating cancer progression, neurodegenerative diseases, and inflammatory responses, where HSP90AB1 levels and modifications are relevant. Researchers utilize this ELISA to quantify HSP90AB1 levels, providing insights into its role in these conditions and potential therapeutic interventions.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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