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Quantitative Human HSP90AB1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX200)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human HSP90AB1 (glycosylated) sandwich ELISA kit is designed to detect human heat shock protein 90kda αB1 (HSP90AB1) levels. HSP90AB1 is a molecular chaperone that plays a critical role in protein folding, stabilization, and signal transduction pathways, influencing cellular responses to stress. Its activity is implicated in various diseases, including cancer and neurodegenerative disorders. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.731 ng/mL, which can accurately detect low concentrations of HSP90AB1 in the sample.
Target HSP90AB1
O-Glycosylation Site 434, 452
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.731 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 2.50 ng/mL-100 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Heat shock protein 90kda αB1
Alternate Names HSP90AB1; Heat shock protein 90kda αB1; HSP90B; HSPCB
Uniprot No. P08238
Application The quantitative human HSP90AB1 (glycosylated) sandwich ELISA kit is valuable in research investigating cancer progression, neurodegenerative diseases, and inflammatory responses, where HSP90AB1 levels and modifications are relevant. Researchers utilize this ELISA to quantify HSP90AB1 levels, providing insights into its role in these conditions and potential therapeutic interventions.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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