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Quantitative Human ICAM4 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX595

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Specifications
Product Description
The quantitative human ICAM4 (glycosylated) sandwich ELISA kit is designed to detect human intercellular adhesion molecule 4 (ICAM4) levels. ICAM4 is expressed on red blood cells, which interacts with integrins. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.093 ng/mL, which can accurately detect low concentrations of ICAM4 in the sample.
Target
ICAM4
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.093 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Intercellular adhesion molecule 4
Alternate Names
ICAM4; Intercellular adhesion molecule 4; CD242; LW; Landsteiner-Wiener blood group glycoprotein
Uniprot No.
N-Glycosylation Site
68, 78, 190, 223
Application Notes
Application
The quantitative human ICAM4 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of ICAM4 concentrations in biological samples. This measurement is important for research focused on cell-cell interactions. It also contributes to understanding the role of ICAM4 in red blood cell adhesion.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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