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Quantitative Human IgG1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX140)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human IgG1 (glycosylated) sandwich ELISA kit is designed to detect human immunoglobulin G1 (IgG1) levels. IgG1 is the most abundant IgG subclass in human serum, playing a critical role in humoral immunity through neutralization, opsonization, and complement activation. It is involved in various immune responses, including defense against pathogens. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 3.138 µg/mL, which can accurately detect low concentrations of IgG1 in the sample.
Target IgG1
N-Glycosylation Site 180
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 3.138 µg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 10 µg/mL-350 µg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Immunoglobulin G1
Alternate Names IgG1; Immunoglobulin G1; IGHG1; Immunoglobulin heavy constant gamma 1
Uniprot No. P01857
Application The quantitative human IgG1 (glycosylated) sandwich ELISA kit is valuable in research and clinical settings for monitoring IgG1 levels in autoimmune diseases, infectious diseases, and during therapeutic interventions with monoclonal antibodies. Researchers and clinicians use this ELISA to quantify IgG1, providing insights into immune status and treatment efficacy.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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