Quantitative Human IgG1 (Glycosylated) Sandwich ELISA Kit

The quantitative human IgG1 (glycosylated) sandwich ELISA kit is designed to detect human immunoglobulin G1 (IgG1) levels. IgG1 is the most abundant IgG subclass in human serum, playing a critical role in humoral immunity through neutralization, opsonization, and complement activation. It is involved in various immune responses, including defense against pathogens. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 3.138 µg/mL, which can accurately detect low concentrations of IgG1 in the sample.

IgG1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

3.138 µg/mL

Quantitative sandwich ELISA

10 µg/mL-350 µg/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Immunoglobulin G1

IgG1; Immunoglobulin G1; IGHG1; Immunoglobulin heavy constant gamma 1

180

The quantitative human IgG1 (glycosylated) sandwich ELISA kit is valuable in research and clinical settings for monitoring IgG1 levels in autoimmune diseases, infectious diseases, and during therapeutic interventions with monoclonal antibodies. Researchers and clinicians use this ELISA to quantify IgG1, providing insights into immune status and treatment efficacy.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-105%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.