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Quantitative Human IL1RAP (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX833

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Specifications
Product Description
The quantitative human IL1RAP (glycosylated) sandwich ELISA kit is designed to detect human interleukin 1 receptor accessory protein (IL1RAP) levels. IL1RAP is a transmembrane protein that is a coreceptor for the interleukin-1 (IL-1) receptor family. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.079 ng/mL, which can accurately detect low concentrations of IL1RAP in the sample.
Target
IL1RAP
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.079 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Interleukin 1 receptor accessory protein
Alternate Names
IL1RAP; Interleukin 1 receptor accessory protein; C3orf13; IL1R3; Interleukin-1 receptor 3
Uniprot No.
N-Glycosylation Site
57, 107, 111, 118, 196, 209, 299
Application Notes
Application
The quantitative human IL1RAP (glycosylated) sandwich ELISA kit is used to measure IL1RAP levels in biological samples. This kit is applicable in studies examining cytokine signaling and inflammatory diseases. It is also relevant to studies investigating the role of IL1RAP in various immune responses.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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