Quantitative Human ISR (Glycosylated) Sandwich ELISA Kit

The quantitative human ISR (glycosylated) sandwich ELISA kit is designed to detect human insulin receptor (ISR) levels. ISR is a transmembrane receptor tyrosine kinase that mediates the biological effects of insulin, regulating glucose metabolism, cell growth, and differentiation. It is crucial for maintaining glucose homeostasis. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.792 ng/mL, which can accurately detect low concentrations of ISR in the sample.

ISR

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.792 ng/mL

Quantitative sandwich ELISA

2.5 ng/mL-100 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Insulin receptor

ISR; Insulin receptor; CD220; INSR; Insulin receptor subunit β

43, 52, 105, 138, 242, 282, 322, 364, 424, 445, 541, 633, 651, 698, 769, 782, 920, 933

The quantitative human ISR (glycosylated) sandwich ELISA kit is valuable in research investigating insulin resistance, diabetes, and related metabolic disorders. Researchers employ this ELISA to quantify ISR levels, providing insights into receptor expression and potential therapeutic targets.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.