Quantitative Human LEPREL1 (Glycosylated) Sandwich ELISA Kit

The quantitative human LEPREL1 (glycosylated) sandwich ELISA kit is designed to detect human leprecan like protein 1 (LEPREL1) levels. LEPREL1 is an enzyme that belongs to the prolyl 3-hydroxylase family. These enzymes catalyze the hydroxylation of proline residues in collagen. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.073 ng/mL, which can accurately detect low concentrations of LEPREL1 in the sample.

LEPREL1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.073 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Leprecan like protein 1

LEPREL1; Leprecan like protein 1; MLAT4; P3H2; Prolyl 3-hydroxylase 2; Myxoid liposarcoma-associated protein 4

449, 549

The quantitative human LEPREL1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated LEPREL1 levels of this enzyme in biological samples. The kit is applicable in studies of collagen biosynthesis, extracellular matrix biology, and connective tissue disorders. It is useful in fields such as cell biology and biochemistry.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 90-105%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.