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Quantitative Human LEPREL1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX678)

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1×48 T
1×96 T

Product Description The quantitative human LEPREL1 (glycosylated) sandwich ELISA kit is designed to detect human leprecan like protein 1 (LEPREL1) levels. LEPREL1 is an enzyme that belongs to the prolyl 3-hydroxylase family. These enzymes catalyze the hydroxylation of proline residues in collagen. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.073 ng/mL, which can accurately detect low concentrations of LEPREL1 in the sample.
Target LEPREL1
N-Glycosylation Site 449, 549
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.073 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Leprecan like protein 1
Alternate Names LEPREL1; Leprecan like protein 1; MLAT4; P3H2; Prolyl 3-hydroxylase 2; Myxoid liposarcoma-associated protein 4
Uniprot No. Q8IVL5
Application The quantitative human LEPREL1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated LEPREL1 levels of this enzyme in biological samples. The kit is applicable in studies of collagen biosynthesis, extracellular matrix biology, and connective tissue disorders. It is useful in fields such as cell biology and biochemistry.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-105%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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