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Quantitative Human MHCDQ β1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX148)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human MHCDQ β1 (glycosylated) sandwich ELISA kit is designed to detect human major histocompatibility complex class 2 DQβ1 (MHCDQ β1) levels. MHCDQ β1 is a subunit of the HLA-DQ molecule, playing a vital role in presenting processed antigens to CD4+ T cells, thus initiating adaptive immune responses. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of MHCDQ β1 in the sample.
Target MHCDQ β1
N-Glycosylation Site 51
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.125 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-20 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Major histocompatibility complex class 2 DQβ1
Alternate Names MHCDQ β1; Major histocompatibility complex class 2 DQβ1; HLADQB1; CELIAC1; HLA class II histocompatibility antigen,DQ Beta 1 chain
Uniprot No. P01920
Application The quantitative human MHCDQ β1 (glycosylated) sandwich ELISA kit is used in research investigating HLA-DQ-associated autoimmune diseases, transplant rejection, and immune responses related to specific HLA-DQ alleles.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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