Quantitative Human MHCDQ β1 (Glycosylated) Sandwich ELISA Kit

The quantitative human MHCDQ β1 (glycosylated) sandwich ELISA kit is designed to detect human major histocompatibility complex class 2 DQβ1 (MHCDQ β1) levels. MHCDQ β1 is a subunit of the HLA-DQ molecule, playing a vital role in presenting processed antigens to CD4+ T cells, thus initiating adaptive immune responses. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of MHCDQ β1 in the sample.

MHCDQ β1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.125 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-20 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Major histocompatibility complex class 2 DQβ1

MHCDQ β1; Major histocompatibility complex class 2 DQβ1; HLADQB1; CELIAC1; HLA class II histocompatibility antigen,DQ Beta 1 chain

51

The quantitative human MHCDQ β1 (glycosylated) sandwich ELISA kit is used in research investigating HLA-DQ-associated autoimmune diseases, transplant rejection, and immune responses related to specific HLA-DQ alleles.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.