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Quantitative Human MHCDRβ1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX147

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Specifications
Product Description
The quantitative human MHCDRβ1 (glycosylated) sandwich ELISA kit is designed to detect human major histocompatibility complex class 2 DRβ1 (MHCDRβ1) levels. MHCDRβ1 is a subunit of the HLA-DR molecule, essential for presenting processed antigens to CD4+ T cells and initiating adaptive immune responses. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 39.016 pg/mL, which can accurately detect low concentrations of MHCDR β1 in the sample.
Target
MHCDR β1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
39.016 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
150 pg/mL-7000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Major histocompatibility complex class 2 DRβ1
Alternate Names
MHCDR β1; Major histocompatibility complex class 2 DRβ1; HLA-DR1B
Uniprot No.
N-Glycosylation Site
48
Application Notes
Application
The quantitative human MHCDR β1 (glycosylated) sandwich ELISA kit is utilized in research investigating HLA-DR-associated autoimmune diseases, transplant rejection, and immune responses related to specific HLA-DR alleles.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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