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Quantitative Human NGF (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX135

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Specifications
Product Description
The quantitative human NGF (glycosylated) sandwich ELISA kit is designed to detect human nerve growth factor (NGF) levels. NGF is a neurotrophin that plays a critical role in the development, maintenance, and survival of neurons. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 6.936 pg/mL, which can accurately detect low concentrations of NGF in the sample.
Target
NGF
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
6.936 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
20 pg/mL-1000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Nerve growth factor
Alternate Names
NGF; Nerve growth factor; NGFB; β-NGF; β-Nerve growth factor
Uniprot No.
N-Glycosylation Site
69, 114
Application Notes
Application
The quantitative human NGF (glycosylated) sandwich ELISA kit is utilized in research investigating the role of NGF in neurological disorders, pain, and inflammation. Researchers employ this ELISA to quantify NGF levels, providing insights into its involvement in these conditions and potential therapeutic applications.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-105%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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