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| Size | Qty | Add To Basket |
|---|---|---|
| 1×48 T | ||
| 1×96 T |
| Product Description | The quantitative human NGF (glycosylated) sandwich ELISA kit is designed to detect human nerve growth factor (NGF) levels. NGF is a neurotrophin that plays a critical role in the development, maintenance, and survival of neurons. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 6.936 pg/mL, which can accurately detect low concentrations of NGF in the sample. |
| Target | NGF |
| N-Glycosylation Site | 69, 114 |
| Sample Types | Tissue homogenates, cell lysates, serum, plasma |
| Sample Volume | 100 μL |
| Sensitivity | 6.936 pg/mL |
| Detection Principle | Quantitative sandwich ELISA |
| Detection Range | 20 pg/mL-1000 pg/mL |
| Detection Time | 1 h-5 h |
| Detection Wavelength | 450 nm |
| Storage | Store at 2-8°C for long term storage. |
| Species | Human |
| Full Name | Nerve growth factor |
| Alternate Names | NGF; Nerve growth factor; NGFB; β-NGF; β-Nerve growth factor |
| Uniprot No. | P01138 |
| Application | The quantitative human NGF (glycosylated) sandwich ELISA kit is utilized in research investigating the role of NGF in neurological disorders, pain, and inflammation. Researchers employ this ELISA to quantify NGF levels, providing insights into its involvement in these conditions and potential therapeutic applications. |
| Kit Components | Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution |
| Precision | Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%; |
| Recovery | Serum sample: n=5, 85-105%; Plasma sample: n=4, 90-105%; |
| Standard Curve | ![]() The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested. |