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Quantitative Human NGF (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX135)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human NGF (glycosylated) sandwich ELISA kit is designed to detect human nerve growth factor (NGF) levels. NGF is a neurotrophin that plays a critical role in the development, maintenance, and survival of neurons. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 6.936 pg/mL, which can accurately detect low concentrations of NGF in the sample.
Target NGF
N-Glycosylation Site 69, 114
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 6.936 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 20 pg/mL-1000 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Nerve growth factor
Alternate Names NGF; Nerve growth factor; NGFB; β-NGF; β-Nerve growth factor
Uniprot No. P01138
Application The quantitative human NGF (glycosylated) sandwich ELISA kit is utilized in research investigating the role of NGF in neurological disorders, pain, and inflammation. Researchers employ this ELISA to quantify NGF levels, providing insights into its involvement in these conditions and potential therapeutic applications.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-105%;
Plasma sample: n=4, 90-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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