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Quantitative Human PDGFB (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX134)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human PDGFB (glycosylated) sandwich ELISA kit is designed to detect human platelet derived growth factor subunit B (PDGFB) levels. PDGFB is a potent growth factor that plays a crucial role in cell proliferation, angiogenesis, and wound healing. It signals through PDGF receptors and is involved in various physiological and pathological processes. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 7.146 pg/mL, which can accurately detect low concentrations of PDGFB in the sample.
Target PDGFB
N-Glycosylation Site 63
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 7.146 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 20 pg/mL-1000 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Platelet derived growth factor subunit B
Alternate Names PDGFB; Platelet derived growth factor subunit B; PDGF2; SIS; Proto-oncogene c-Sis
Uniprot No. P01127
Application The quantitative human PDGFB (glycosylated) sandwich ELISA kit is utilized in research investigating the role of PDGFB in diseases like cancer, atherosclerosis, and fibrosis. Researchers use this ELISA to quantify PDGFB levels, aiding in the understanding of its involvement in these conditions and the development of potential therapeutic strategies.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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