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Quantitative Human PDGFB (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX134

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Specifications
Product Description
The quantitative human PDGFB (glycosylated) sandwich ELISA kit is designed to detect human platelet derived growth factor subunit B (PDGFB) levels. PDGFB is a potent growth factor that plays a crucial role in cell proliferation, angiogenesis, and wound healing. It signals through PDGF receptors and is involved in various physiological and pathological processes. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 7.146 pg/mL, which can accurately detect low concentrations of PDGFB in the sample.
Target
PDGFB
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
7.146 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
20 pg/mL-1000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Platelet derived growth factor subunit B
Alternate Names
PDGFB; Platelet derived growth factor subunit B; PDGF2; SIS; Proto-oncogene c-Sis
Uniprot No.
N-Glycosylation Site
63
Application Notes
Application
The quantitative human PDGFB (glycosylated) sandwich ELISA kit is utilized in research investigating the role of PDGFB in diseases like cancer, atherosclerosis, and fibrosis. Researchers use this ELISA to quantify PDGFB levels, aiding in the understanding of its involvement in these conditions and the development of potential therapeutic strategies.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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