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Quantitative Human PFKP (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX514)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human PFKP (glycosylated) sandwich ELISA kit is designed to detect human phosphofructokinase (PFKP) levels. PFKP is an enzyme that plays a key regulatory role in glycolysis. Glycolysis is the metabolic pathway that breaks down glucose to produce energy. PFKP catalyzes a critical step in this pathway, and its activity is tightly regulated. This regulation helps control the rate of glucose metabolism in response to cellular energy demands. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.120 ng/mL, which can accurately detect low concentrations of PFKP in the sample.
Target PFKP
O-Glycosylation Site 540
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.120 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Phosphofructokinase
Alternate Names PFKP; Phosphofructokinase; PFK-C; 6-Phosphofructokinase type C; Phosphofructo-1-kinase isozyme C
Uniprot No. Q01813
Application The quantitative human PFKP (glycosylated) sandwich ELISA kit is a valuable tool for research in biochemistry and metabolism, particularly in studies of glycolysis and energy metabolism. Researchers use it to measure PFKP levels in biological samples. The kit has applications in studies of metabolic disorders, muscle diseases, and platelet functions.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-95%;
Plasma sample: n=4, 80-90%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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