Quantitative Human PFKP (Glycosylated) Sandwich ELISA Kit

The quantitative human PFKP (glycosylated) sandwich ELISA kit is designed to detect human phosphofructokinase (PFKP) levels. PFKP is an enzyme that plays a key regulatory role in glycolysis. Glycolysis is the metabolic pathway that breaks down glucose to produce energy. PFKP catalyzes a critical step in this pathway, and its activity is tightly regulated. This regulation helps control the rate of glucose metabolism in response to cellular energy demands. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.120 ng/mL, which can accurately detect low concentrations of PFKP in the sample.

PFKP

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.120 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Phosphofructokinase

PFKP; Phosphofructokinase; PFK-C; 6-Phosphofructokinase type C; Phosphofructo-1-kinase isozyme C

540

The quantitative human PFKP (glycosylated) sandwich ELISA kit is a valuable tool for research in biochemistry and metabolism, particularly in studies of glycolysis and energy metabolism. Researchers use it to measure PFKP levels in biological samples. The kit has applications in studies of metabolic disorders, muscle diseases, and platelet functions.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-95%;
Plasma sample: n=4, 80-90%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.