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Quantitative Human PIA (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX495

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative human PIA (glycosylated) sandwich ELISA kit is designed to detect human peptidylprolyl isomerase A (PIA) levels. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.502 ng/mL, which can accurately detect low concentrations of PIA in the sample.
Target
PIA
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.502 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
2 ng/mL-60 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Peptidylprolyl isomerase A
Alternate Names
PIA; Peptidylprolyl isomerase A; PPIA; CYPH; Peptidyl-prolyl isomerase A; Cyclosporin A-binding protein
Uniprot No.
P62937
N-Glycosylation Site
108
Application Notes
Application
The quantitative human PIA (glycosylated) sandwich ELISA kit is used to quantify PIA in biological samples. This quantification could be useful in research studying the protein's functions, its role in diseases, or its response to treatments.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-110%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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