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Quantitative Human PLA2G2D (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX898)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human PLA2G2D (glycosylated) sandwich ELISA kit is designed to detect human phospholipase A2, group 2D (PLA2G2D) levels. PLA2G2D is an enzyme that belongs to the phospholipase A2 superfamily. This enzyme catalyzes the hydrolysis of membrane phospholipids, releasing fatty acids like arachidonic acid. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.135 ng/mL, which can accurately detect low concentrations of PLA2G2D in the sample.
Target PLA2G2D
N-Glycosylation Site 89
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.135 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Phospholipase A2, group 2D
Alternate Names PLA2G2D; Phospholipase A2, group 2D; sPLA2; SPLASH; Secreted phospholipase A2
Uniprot No. Q9UNK4
Application The quantitative human PLA2G2D (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated PLA2G2D levels in biological samples. The assay can be utilized to investigate the role of PLA2G2D in various physiological and pathological conditions, including inflammatory diseases, and cellular signaling pathways.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-110%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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