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Quantitative Human PSGL1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX586)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human PSGL1 (glycosylated) sandwich ELISA kit is designed to detect human p-Selectin glycoprotein ligand 1 (PSGL1) levels. PSGL1 is a protein that plays a critical role in leukocyte adhesion. PSGL1 interacts with selectins, a family of cell adhesion molecules. This interaction is essential for the initial steps of leukocyte migration. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.553 ng/mL, which can accurately detect low concentrations of PSGL1 in the sample.
Target PSGL1
N-Glycosylation Site 65, 111, 302
O-Glycosylation Site 57
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.553 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 2 ng/mL-35 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name P-Selectin glycoprotein ligand 1
Alternate Names PSGL1; P-Selectin glycoprotein ligand 1; CD162; SELPLG; Selectin P ligand
Uniprot No. Q14242
Application The quantitative human PSGL1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of PSGL-1 levels in biological samples. This measurement is crucial for studies investigating leukocyte adhesion. It also contributes to understanding the mechanisms of inflammation.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-110%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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