Quantitative Human RANKL (Glycosylated) Sandwich ELISA Kit

The quantitative human RANKL (glycosylated) sandwich ELISA kit is designed to detect human receptor activator of nuclear factor-KB ligand (RANKL) levels. RANKL is a cytokine that plays a crucial role in osteoclast differentiation and activation, regulating bone remodeling and immune responses. It is essential for bone metabolism and immune cell interactions. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 25.148 pg/mL, which can accurately detect low concentrations of RANKL in the sample.

RANKL

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

25.148 pg/mL

Quantitative sandwich ELISA

80 pg/mL-4000 pg/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Receptor activator of nuclear factor-KB ligand

RANKL; Receptor activator of nuclear factor-KB ligand; CD254; TNFSF11; Tumor necrosis factor (ligand) superfamily member 11; TNF-related activation-induced cytokine

171, 198

The quantitative human RANKL (glycosylated) sandwich ELISA kit is valuable for researchers investigating osteoporosis, rheumatoid arthritis, and cancer-induced bone diseases, where RANKL levels are often altered. By quantifying RANKL, researchers can gain insights into disease pathogenesis and explore potential therapeutic targets related to bone metabolism and immune regulation.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 95-110%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.