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Quantitative Human RHO (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX194)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human RHO (glycosylated) sandwich ELISA kit is designed to detect human rhodopsin (RHO) levels. RHO is a light-sensitive G protein-coupled receptor located in the rod cells of the retina, essential for vision in low-light conditions. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.766 ng/mL, which can accurately detect low concentrations of RHO in the sample.
Target RHO
N-Glycosylation Site 2, 15
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.766 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 2.5 ng/mL-100 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Rhodopsin
Alternate Names RHO; Rhodopsin; OPN2; RP4; Opsin 2; Retinitis pigmentosa 4
Uniprot No. P08100
Application The quantitative human RHO (glycosylated) sandwich ELISA kit is valuable in research investigating retinal diseases such as retinitis pigmentosa, night blindness, and other vision-related disorders. Researchers utilize this ELISA to quantify RHO levels, providing insights into its expression and potential diagnostic or therapeutic applications related to visual function.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 95-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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