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Quantitative Human SEMA3E (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX35)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human SEMA3E (glycosylated) sandwich ELISA kit is designed to detect human semaphorin 3E (SEMA3E) levels. SEMA3E is a secreted protein that plays a role in axonal guidance, angiogenesis, and tumor suppression by interacting with its receptor, Plexin D1. It is involved in cell migration, adhesion, and signaling pathways. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.110 ng/mL, which can accurately detect low concentrations of SEMA3E in the sample.
Target SEMA3E
N-Glycosylation Site 44, 126, 330, 595, 596
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.110 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Semaphorin 3E
Alternate Names SEMA3E; Semaphorin 3E; SEMAH; Coll-5
Uniprot No. O15041
Application The quantitative human SEMA3E (glycosylated) sandwich ELISA kit is valuable for researchers investigating cancer, vascular diseases, and neurological disorders, where SEMA3E signaling is implicated. By quantifying SEMA3E, researchers can gain insights into its role in disease pathogenesis and explore potential therapeutic targets related to cell migration and angiogenesis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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