Quantitative Human SEMA3E (Glycosylated) Sandwich ELISA Kit

The quantitative human SEMA3E (glycosylated) sandwich ELISA kit is designed to detect human semaphorin 3E (SEMA3E) levels. SEMA3E is a secreted protein that plays a role in axonal guidance, angiogenesis, and tumor suppression by interacting with its receptor, Plexin D1. It is involved in cell migration, adhesion, and signaling pathways. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.110 ng/mL, which can accurately detect low concentrations of SEMA3E in the sample.

SEMA3E

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

0.110 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-15 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Semaphorin 3E

SEMA3E; Semaphorin 3E; SEMAH; Coll-5

44, 126, 330, 595, 596

The quantitative human SEMA3E (glycosylated) sandwich ELISA kit is valuable for researchers investigating cancer, vascular diseases, and neurological disorders, where SEMA3E signaling is implicated. By quantifying SEMA3E, researchers can gain insights into its role in disease pathogenesis and explore potential therapeutic targets related to cell migration and angiogenesis.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.