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Quantitative Human SFRP1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX1001

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative human SFRP1 (glycosylated) sandwich ELISA kit is designed to detect human secreted frizzled-related protein 1 (SFRP1)) levels. It is suitable for various biological samples such as tissue homogenates, cell culture supernates, serum, or plasma. Its sensitivity is 0.826 pg/mL, which can accurately detect low concentrations of SFRP1 in the sample.
Target
SFRP1
Kit Components
Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated SFRP1 antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell culture supernates, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
0.826 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
3.25 pg/mL-200 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Secreted frizzled-related protein 1
Alternate Names
Frizzled related protein 1; FRP1; SARP2; Secreted apoptosis-related protein 2; Secreted frizzled related protein 1; Secreted frizzled related protein; SFRP1
Uniprot No.
Q8N474
N-Glycosylation Site
173
Application Notes
Application
The quantitative human SFRP1 (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of SFRP1, helping to understand its role in cell biology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-105%;
Plasma sample: n=4, 80-90%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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