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Quantitative Human SI (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX247)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human SI (glycosylated) sandwich ELISA kit is designed to detect human sucrase isomaltase (SI) levels. SI is an intestinal brush border enzyme that hydrolyzes sucrose and isomaltose, playing a crucial role in carbohydrate digestion. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of SI in the sample.
Target SI
N-Glycosylation Site 99, 437, 455, 823, 855, 904, 926, 1235, 1303, 1340, 1354, 1403, 1535, 1572, 1675, 1748, 1763, 1815
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.125 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Sucrase isomaltase
Alternate Names SI; Sucrase isomaltase
Uniprot No. P14410
Application The quantitative human SI (glycosylated) sandwich ELISA kit is designed to measure the levels of glycosylated SI in samples, aiding in the diagnosis and study of carbohydrate malabsorption disorders and intestinal diseases. This kit allows researchers to quantify SI levels, providing insights into intestinal function and related pathologies.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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