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Quantitative Human SI (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX247

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Specifications
Product Description
The quantitative human SI (glycosylated) sandwich ELISA kit is designed to detect human sucrase isomaltase (SI) levels. SI is an intestinal brush border enzyme that hydrolyzes sucrose and isomaltose, playing a crucial role in carbohydrate digestion. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.125 ng/mL, which can accurately detect low concentrations of SI in the sample.
Target
SI
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.125 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Sucrase isomaltase
Alternate Names
SI; Sucrase isomaltase
Uniprot No.
N-Glycosylation Site
99, 437, 455, 823, 855, 904, 926, 1235, 1303, 1340, 1354, 1403, 1535, 1572, 1675, 1748, 1763, 1815
Application Notes
Application
The quantitative human SI (glycosylated) sandwich ELISA kit is designed to measure the levels of glycosylated SI in samples, aiding in the diagnosis and study of carbohydrate malabsorption disorders and intestinal diseases. This kit allows researchers to quantify SI levels, providing insights into intestinal function and related pathologies.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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