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Quantitative Human Siglec-5 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX40)

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1×48 T
1×96 T

Product Description The quantitative human siglec-5 (glycosylated) sandwich ELISA kit is designed to detect human sialic acid-binding Ig-like lectin 5 (Siglec5) levels. Siglec-5 is a transmembrane protein that binds to sialic acid-containing ligands, playing a role in regulating immune responses and cell signaling. It is involved in inhibitory signaling pathways that modulate immune cell activation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 30.182 pg/mL, which can accurately detect low concentrations of siglec-5 in the sample.
Target Siglec-5
N-Glycosylation Site 100, 210, 231, 253, 328, 375, 384, 393
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 30.182 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 80 pg/mL-4000 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Sialic acid-binding Ig-like lectin 5
Alternate Names Siglec-5; Sialic acid-binding Ig-like lectin 5; CD170; OBBP2; SIGLEC-5
Uniprot No. O15389
Application The quantitative human siglec-5 (glycosylated) sandwich ELISA kit is useful for researchers investigating inflammatory diseases, autoimmune disorders, and cancer, where siglec-5 expression and function are relevant. By quantifying siglec-5, researchers can gain insights into its role in immune regulation and explore potential therapeutic targets related to immune modulation.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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