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Quantitative Human SIGLEC14 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX541

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Specifications
Product Description
The quantitative human SIGLEC14 (glycosylated) sandwich ELISA kit is designed to detect human sialic acid binding Ig like lectin 14 (SIGLEC14) levels. SIGLEC14 is a protein that belongs to the Siglec family. SIGLEC14 is expressed on immune cells, including neutrophils and monocytes, which is involved in regulating immune responses and inflammation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 14.025 pg/mL, which can accurately detect low concentrations of SIGLEC14 in the sample.
Target
SIGLEC14
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
14.025 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
50 pg/mL-1500 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Sialic acid binding Ig like lectin 14
Alternate Names
SIGLEC14; Sialic acid binding Ig like lectin 14; SIG14_HUMAN; Siglec-14
Uniprot No.
N-Glycosylation Site
231
Application Notes
Application
The quantitative human SIGLEC14 (glycosylated) sandwich ELISA kit is a valuable tool for research in immunology and inflammation, particularly in studies of Siglec biology. Researchers use it to measure SIGLEC14 levels in immune cells and biological fluids. The kit has applications in studies of inflammatory diseases and immune regulation.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 95-110%;
Plasma sample: n=4, 95-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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