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Quantitative Human SIGLEC14 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX541)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human SIGLEC14 (glycosylated) sandwich ELISA kit is designed to detect human sialic acid binding Ig like lectin 14 (SIGLEC14) levels. SIGLEC14 is a protein that belongs to the Siglec family. SIGLEC14 is expressed on immune cells, including neutrophils and monocytes, which is involved in regulating immune responses and inflammation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 14.025 pg/mL, which can accurately detect low concentrations of SIGLEC14 in the sample.
Target SIGLEC14
N-Glycosylation Site 231
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 14.025 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 50 pg/mL-1500 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Sialic acid binding Ig like lectin 14
Alternate Names SIGLEC14; Sialic acid binding Ig like lectin 14; SIG14_HUMAN; Siglec-14
Uniprot No. Q08ET2
Application The quantitative human SIGLEC14 (glycosylated) sandwich ELISA kit is a valuable tool for research in immunology and inflammation, particularly in studies of Siglec biology. Researchers use it to measure SIGLEC14 levels in immune cells and biological fluids. The kit has applications in studies of inflammatory diseases and immune regulation.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 95-110%;
Plasma sample: n=4, 95-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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