Quantitative Human SIGLEC8 (Glycosylated) Sandwich ELISA Kit

The quantitative human SIGLEC8 (glycosylated) sandwich ELISA kit is designed to detect human sialic acid binding Ig like lectin 8 (SIGLEC8) levels. SIGLEC8 is a transmembrane protein that is expressed on eosinophils, mast cells, and basophils. SIGLEC8 plays a role in regulating allergic inflammation. The kit is suitable for various biological samples such as cell lysates. Its sensitivity is 0.066 ng/mL, which can accurately detect low concentrations of SIGLEC8 in the sample.

SIGLEC8

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Cell lysates

100 μL

0.066 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Sialic acid binding Ig like lectin 8

SIGLEC8; Sialic acid binding Ig like lectin 8; SAF2; Sialoadhesin family member 2

172, 249, 267

The quantitative human SIGLEC8 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of SIGLEC8 in biological samples. This kit is applicable in studies examining allergic inflammation and asthma. It is also relevant to studies investigating the role of SIGLEC8 in modulating the activity of eosinophils, mast cells, and basophils, and its potential as a therapeutic target for allergic diseases.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.