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Quantitative Human SIGLEC8 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX859)

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1×48 T
1×96 T

Product Description The quantitative human SIGLEC8 (glycosylated) sandwich ELISA kit is designed to detect human sialic acid binding Ig like lectin 8 (SIGLEC8) levels. SIGLEC8 is a transmembrane protein that is expressed on eosinophils, mast cells, and basophils. SIGLEC8 plays a role in regulating allergic inflammation. The kit is suitable for various biological samples such as cell lysates. Its sensitivity is 0.066 ng/mL, which can accurately detect low concentrations of SIGLEC8 in the sample.
Target SIGLEC8
N-Glycosylation Site 172, 249, 267
Sample Types Cell lysates
Sample Volume 100 μL
Sensitivity 0.066 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Sialic acid binding Ig like lectin 8
Alternate Names SIGLEC8; Sialic acid binding Ig like lectin 8; SAF2; Sialoadhesin family member 2
Uniprot No. Q9NYZ4
Application The quantitative human SIGLEC8 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of SIGLEC8 in biological samples. This kit is applicable in studies examining allergic inflammation and asthma. It is also relevant to studies investigating the role of SIGLEC8 in modulating the activity of eosinophils, mast cells, and basophils, and its potential as a therapeutic target for allergic diseases.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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