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Quantitative Human TNFRSF5 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX336

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Specifications
Product Description
The quantitative human TNFRSF5 (glycosylated) sandwich ELISA kit is designed to detect human tumor necrosis factor receptor superfamily, member 5 (TNFRSF5) levels. TNFRSF5, also known as CD40, is a transmembrane protein. It is a receptor for CD40 ligand. TNFRSF5 plays a role in B cell activation. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 4.027 pg/mL, which can accurately detect low concentrations of TNFRSF5 in the sample.
Target
TNFRSF5
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
4.027 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
20 pg/mL-900 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Tumor necrosis factor receptor superfamily, member 5
Alternate Names
TNFRSF5; Tumor necrosis factor receptor superfamily, member 5; CD40; Bp50; B-cell surface antigen CD40
Uniprot No.
N-Glycosylation Site
153, 180
Application Notes
Application
The quantitative human TNFRSF5 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated TNFRSF5 levels. It is useful for studying the role of CD40 in B cell activation.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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