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Quantitative Human UNC5B (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX685

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Specifications
Product Description
The quantitative human UNC5B (glycosylated) sandwich ELISA kit is designed to detect human netrin receptor (UNC5B) levels. UNC5B is a receptor, which is involved in axon guidance. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.073 ng/mL, which can accurately detect low concentrations of UNC5B in the sample.
Target
UNC5B
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.073 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Netrin receptor
Alternate Names
UNC5B; Netrin receptor; Netrin receptor UNC5B; Protein unc-5 homolog B; UNC5H2
Uniprot No.
N-Glycosylation Site
222, 347
Application Notes
Application
The quantitative human UNC5B (glycosylated) sandwich ELISA kit is used to determine UNC5B levels in biological specimens. This measurement is crucial for studies investigating nervous system development. It also contributes to understanding the role of UNC5B in axon guidance. Furthermore, it is valuable in research exploring neurological disorders and developmental processes.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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