Quantitative Human WNT5A (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0325-HX1228)

Product Description	The quantitative human WNT5A (glycosylated) sandwich ELISA kit is designed to detect human wingless-type MMTV integration site family, member 5A (WNT5A) levels. It is suitable for various biological samples such as tissue homogenates, serum, or plasma. Its sensitivity is 0.044 ng/mL, which can accurately detect low concentrations of WNT5A in the sample.
Target	WNT5A
N-Glycosylation Site	114, 120, 312, 326
Sample Types	Tissue homogenates, serum, plasma
Sample Volume	50 μL-100 μL
Sensitivity	0.044 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	0.16 ng/mL-10 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Wingless-type MMTV integration site family, member 5A
Alternate Names	Wingless-type MMTV integration site family, member 5A; hWNT5A; Protein Wnt-5a; WNT 5A protein precursor; WNT5A
Uniprot No.	P41221

Application	The quantitative human WNT5A (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of WNT5A, helping to understand its role in stem cells.
Kit Components	Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated WNT5A antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 80-100%; Plasma sample: n=4, 90-105%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.