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Quantitative Mouse LIPA (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX932)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative mouse LIPA (glycosylated) sandwich ELISA kit is designed to detect mouse lipase A (LIPA) levels. LIPA is an enzyme that is located in lysosomes. It is responsible for the hydrolysis of triglycerides and cholesteryl esters. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.158 ng/mL, which can accurately detect low concentrations of LIPA in the sample.
Target LIPA
N-Glycosylation Site 34, 99, 159, 271, 319
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.158 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Mouse
Full Name Lipase A
Alternate Names LIPA; Lipase A; LAL; CESD; Acid cholesteryl ester hydrolase
Uniprot No. Q9Z0M5
Application The quantitative mouse LIPA (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated LIPA levels in biological samples. The assay is valuable for investigating the role of LIPA in lipid metabolism, lysosomal storage disorders, and related metabolic conditions.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 95-105%;
Plasma sample: n=4, 95-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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