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Quantitative Mouse LIPA (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX932

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Specifications
Product Description
The quantitative mouse LIPA (glycosylated) sandwich ELISA kit is designed to detect mouse lipase A (LIPA) levels. LIPA is an enzyme that is located in lysosomes. It is responsible for the hydrolysis of triglycerides and cholesteryl esters. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.158 ng/mL, which can accurately detect low concentrations of LIPA in the sample.
Target
LIPA
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.158 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Mouse
Full Name
Lipase A
Alternate Names
LIPA; Lipase A; LAL; CESD; Acid cholesteryl ester hydrolase
Uniprot No.
N-Glycosylation Site
34, 99, 159, 271, 319
Application Notes
Application
The quantitative mouse LIPA (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated LIPA levels in biological samples. The assay is valuable for investigating the role of LIPA in lipid metabolism, lysosomal storage disorders, and related metabolic conditions.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 95-105%;
Plasma sample: n=4, 95-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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