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GlycoErase™ PHKB Knockout HepG2 Cell Line

CAT#: GLJF-0825-JF669

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Overview Specification Target Information Related Products
Overview
Description
GlycoErase™ PHKB knockout HepG2 cell line eliminates PHKB, which encodes the beta regulatory subunit of phosphorylase kinase, expressed in both liver and muscle. Mutations cause glycogen storage disease type IXB. This knockout supports research into regulatory control of glycogen breakdown, metabolic defects, and calcium-dependent signaling in liver cells.
Product Type
KO Cell Lines
Species
Human
Cell Morphology
Epithelial-like, adherent
Passage Ratio
1:4~1:6
Cell Line
HepG2
Primary Disease
Hepatoblastoma
Lineage
Liver
Lineage Subtype
Hepatoblastoma
Specification
Cell Viability
>90%
Sterility Test
The sterility test indicated an absence of microbial growth.
Identity Test
STR identification
Mycoplasma Test
Negative
Virus Test
Negative for HIV, HBV and HCV.
Genetic Stability Testing
We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation
PCR, Sanger Sequencing
Culture Medium
DMEM & FBS & Glutamine
Application
Functional assay
Size
1 M cells/vial*2
Product Format
Frozen
Shipping
Dry ice
Availability Status
Made to order
Handling Notes
Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer
This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.
Target Information
Target
PHKB
Full Name
Phosphorylase Kinase Regulatory Subunit Beta
Location
16q12.1
Gene ID
5257
Summary
This multi-subunit kinase contains the same beta subunit in both liver and muscle isoforms, and it is encoded by this gene. The gamma unit provides enzymatic activity, with the delta (calmodulin) regulating calcium sensitivity. Mutations are linked to glycogen storage disease type 9B. Two pseudogenes are located on chromosomes 14 and 20.
For Research Use Only.
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