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GlycoErase™ PHKB Knockout HEK293 Cell Line

CAT#: GLJF-0825-JF437

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Overview Specification Target Information Related Products
Overview
Description
GlycoErase™ PHKB knockout HEK293 cell line is a HEK293 model with PHKB expression disrupted. PHKB encodes the beta regulatory subunit of phosphorylase kinase, expressed in both liver and muscle, and mutations cause glycogen storage disease type IXB. This knockout supports research on regulatory glycogen metabolism, multisystem metabolic defects, and calcium-mediated enzyme signaling within a human-derived cellular background.
Product Type
KO Cell Lines
Species
Human
Cell Morphology
Epithelial-like, adherent
Passage Ratio
1:2~1:4
Cell Line
HEK293
Lineage
Embryonic kidney
Specification
Cell Viability
>90%
Sterility Test
The sterility test indicated an absence of microbial growth.
Identity Test
STR identification
Mycoplasma Test
Negative
Virus Test
Negative for HIV, HBV and HCV.
Genetic Stability Testing
We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation
PCR, Sanger Sequencing
Culture Medium
FBS & Penicillin/Streptomycin & Proline & RPMI
Application
Functional assay
Size
1 M cells/vial*2
Product Format
Frozen
Shipping
Dry ice
Availability Status
Made to order
Handling Notes
Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer
This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.
Target Information
Target
PHKB
Full Name
Phosphorylase Kinase Regulatory Subunit Beta
Location
16q12.1
Gene ID
5257
Summary
Phosphorylase kinase is a 16-subunit enzyme containing alpha, beta, gamma, and delta subunits. The alpha subunit encodes muscle and liver isoforms via separate genes. The beta subunit, encoded by this gene, is the same in both forms. The gamma subunit contains the catalytic site, and the delta subunit (a calmodulin) controls calcium sensitivity. Mutations cause glycogen storage disease type 9B, affecting liver and muscle. Pseudogenes are found on chromosomes 14 and 20.
For Research Use Only.
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