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GlycoFlux™ Lenti-Human AMY1A Viral Particle (CAT#: GLJF-1025-JF148)

Datasheet

SPECIFIC INQUIRY
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Titer:
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Description GlycoFlux™ Lenti-AMY1A Viral Particle expresses AMY1A, a salivary α-amylase that initiates starch/glycogen digestion by hydrolyzing 1,4-α-glucosidic bonds; the pre-assembled lentiviral tool facilitates stable cell-line establishment in mammalian cells to examine secretory enzyme biology, gene-copy variation effects, and substrate preference, with routine selection or marker-guided enrichment.
Product Type Recombinant Lentivirus
Target AMY1A
Species Human
Promotor EF1a
Packaging System Lentivirus
Packaging Cell HEK293T
Application The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.
Shipping Shipped on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to maintain their titer and stability.
Handling Notes Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.
Full Name Amylase Alpha 1A
Alternative Name AMY1
Location 1p21.1
Gene ID 276
Summary This salivary gland amylase isoenzyme hydrolyzes 1,4-alpha-glucoside bonds in carbohydrates, initiating starch and glycogen digestion. It is part of a gene cluster expressed at high levels in salivary glands or pancreas. Alternative splicing generates identical protein products.
For Research Use Only.
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