Specifications Quality Control Target Information Related Products

Specifications

Description

GlycoFlux™ Lenti-AMY1A Viral Particle expresses AMY1A, a salivary α-amylase that initiates starch/glycogen digestion by hydrolyzing 1,4-α-glucosidic bonds; the pre-assembled lentiviral tool facilitates stable cell-line establishment in mammalian cells to examine secretory enzyme biology, gene-copy variation effects, and substrate preference, with routine selection or marker-guided enrichment.

Product Type

Recombinant Lentivirus

Target

AMY1A

Species

Human

Promotor

EF1a

Packaging System

Lentivirus

Packaging Cell

HEK293T

Application

The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.

Shipping

Shipped on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to maintain their titer and stability.

Handling Notes

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Quality Control

Titer Assay

qPCR

Mycoplasma Test

Negative

Sterility

Sterility testing confirmed no microbial contamination.

Transduction Evaluation

On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.

Insert Identity Confirmation

All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.

Target Information

Full Name

Amylase Alpha 1A

Alternative Name

AMY1

Location

1p21.1

Gene ID

276

Summary

This salivary gland amylase isoenzyme hydrolyzes 1,4-alpha-glucoside bonds in carbohydrates, initiating starch and glycogen digestion. It is part of a gene cluster expressed at high levels in salivary glands or pancreas. Alternative splicing generates identical protein products.

GlycoFlux™ AAV1-Human AMY1A Viral Particle (CAT#: GLJF-1025-JF919)
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GlycoFlux™ Lenti-Human AMY1A Viral Particle