Description

GlycoFlux™ Lenti-PHKA2 Viral Particle delivers the phosphorylase kinase α (liver) subunit, part of the 16-subunit enzyme where γ is catalytic and δ (calmodulin) confers Ca²⁺ dependence; defects lead to GSD IXa. This platform facilitates stable line generation in mammalian cells for hepatic glycogen turnover models, regulatory mapping, and splice-variant evaluation, with straightforward antibiotic selection or fluorescence sorting.

Product Type

Recombinant Lentivirus

Target

PHKA2

Species

Human

Promotor

EF1a

Packaging System

Lentivirus

Packaging Cell

HEK293T

Application

The lentiviral vector is a powerful, HIV-based system used for gene delivery, notable for its ability to transduce both dividing and non-dividing cells (e.g., neurons, stem cells). It permanently integrates the gene into the host genome, ensuring long-term, sustained expression for applications in research and studies related to gene therapy.

Shipping

Shipped on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to maintain their titer and stability.

Handling Notes

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Titer Assay	qPCR
Mycoplasma Test	Negative
Sterility	Sterility testing confirmed no microbial contamination.
Transduction Evaluation	On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation	All lentiviral vectors at Creative Biolabs are verified for correct proviral integration by PCR. In this assay, cells are transduced with serial dilutions of the lentiviral vector, harvested after a few days, and genomic DNA is isolated. A defined region of the expected lentiviral insert is then PCR-amplified to confirm identity.

Full Name	Phosphorylase Kinase Regulatory Subunit Alpha 2
Alternative Name	PHK; PYK; XLG; PYKL; XLG2; GSD9A
Location	Xp22.13
Gene ID	5256
Summary	Phosphorylase kinase is a 16-subunit enzyme composed of four alpha, beta, gamma, and delta subunits. The alpha subunit includes skeletal muscle and hepatic isoforms, with the hepatic form encoded by this gene. The gamma subunit holds the active site, while alpha and beta are regulatory. The delta subunit, a calmodulin, mediates calcium dependence. Mutations cause glycogen storage disease type 9A (X-linked liver glycogenosis). Alternative splice variants have been reported, though full-length forms are not defined.

GlycoFlux™ Lenti-Human PHKA2 Viral Particle (CAT#: GLJF-1025-JF204)

Specifications

Quality Control

Target Information

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