GlycoFlux™ MMLV-Human PHKA2 Viral Particle

GlycoFlux™ MMLV-PHKA2 Viral Particle delivers PHKA2, the hepatic α regulatory subunit (GSD IXa); optimized to build stable mammalian lines, it supports liver-biased glycogen breakdown models and phospho-regulatory mapping.

Recombinant MMLV Retrovirus

PHKA2

Human

MMLV 5' LTR promoter

Retrovirus

HEK293T

This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.

Shipped on dry ice with temperature held at or below -80°C.

Store at -80°C to maintain their titer and stability.

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

qPCR

Negative

Sterility testing confirmed no microbial contamination.

On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.

Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.

Phosphorylase Kinase Regulatory Subunit Alpha 2

PHK; PYK; XLG; PYKL; XLG2; GSD9A

Xp22.13

Phosphorylase kinase is a 16-subunit enzyme containing four alpha, beta, gamma, and delta units. The hepatic alpha isoform is encoded by this gene, while the gamma unit carries the active site and the delta subunit (calmodulin) regulates calcium sensitivity. Mutations in this gene result in glycogen storage disease type 9A (X-linked liver glycogenosis). Alternative transcripts have been observed but not fully characterized.