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GlycoFlux™ MSCV-Human PHKA2 Viral Particle (CAT#: GLJF-1025-JF718)

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Description GlycoFlux™ MSCV-PHKA2 Viral Particle secures persistent hepatic α subunit (PHKA2) expression to model liver-specific phosphorylase kinase regulation. Suited to GSD-IXA studies and fasting/refeeding paradigms; track PhK activity, hepatic glycogen breakdown, and glucose output.
Product Type Recombinant MSCV Retrovirus
Target PHKA2
Species Human
Promotor MSCV 5' LTR promoter
Packaging System Retrovirus
Packaging Cell HEK293T
Application This MSCV vector is a common retroviral vector system used to achieve high-efficiency, stable gene delivery into target cells, such as hematopoietic stem cells. It ensures long-term, sustained expression by stably integrating the gene into the host cell's genome.
Shipping Dispatched on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to preserve titer and stability.
Handling Notes Store at -80°C and avoid repeated freeze-thaw cycles to preserve viral titer. For safety, handle materials in a biological safety cabinet while wearing appropriate PPE (lab coat, gloves, and eye protection).
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation Creative Biolabs offers, upon request, in vitro and in vivo transduction assays to verify lentiviral targeting and to quantify transgene expression and functional output.
Insert Identity Confirmation All Creative Biolabs retroviral vectors undergo PCR-based verification of proviral integration. Cells are transduced across a dilution series of the retroviral preparation; after several days, genomic DNA is isolated, and a defined region of the expected insert is PCR-amplified to confirm correct identity and integration.
Full Name Phosphorylase Kinase Regulatory Subunit Alpha 2
Alternative Name PHK; PYK; XLG; PYKL; XLG2; GSD9A
Location Xp22.13
Gene ID 5256
Summary Phosphorylase kinase is assembled from 16 total subunits-four each of alpha, beta, gamma, and delta types. In this form, the hepatic alpha isoform is produced by this gene, while the gamma unit hosts the catalytic site. The delta subunit is a calmodulin that manages calcium dependence. Variants in this gene cause glycogen storage disease type 9A. Several splice forms have been detected but remain incompletely characterized.
For Research Use Only.
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