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GlycoFlux™ MMLV-Human C1GALT1C1 Viral Particle (CAT#: GLJF-1025-JF284)

Datasheet

SPECIFIC INQUIRY
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Description GlycoFlux™ MMLV-C1GALT1C1 Viral Particle provides C1GALT1C1 (Cosmc), the folding chaperone for C1GALT1 required to synthesize the core-1 O-glycan (Thomsen-Friedenreich antigen); the vector is intended for generating stable mammalian cell lines to restore core-1 biosynthesis, assess chaperone mutants, and stabilize C1GALT1 activity.
Product Type Recombinant MMLV Retrovirus
Target C1GALT1C1
Species Human
Promotor MMLV 5' LTR promoter
Packaging System Retrovirus
Packaging Cell HEK293T
Application This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.
Shipping Shipped on dry ice with temperature held at or below -80°C.
Storage Store at -80°C to maintain their titer and stability.
Handling Notes Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.
Product Disclaimer These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.
Titer Assay qPCR
Mycoplasma Test Negative
Sterility Sterility testing confirmed no microbial contamination.
Transduction Evaluation On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.
Insert Identity Confirmation Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.
Full Name C1GALT1 specific chaperone 1
Alternative Name C1GALT2; COSMC
Location Xq24
Gene ID 29071
Summary Acts as a folding chaperone for core 1 beta1,3-galactosyltransferase 1, enabling synthesis of the core-1 O-glycan (Thomsen-Friedenreich antigen).
For Research Use Only.
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