Specifications Quality Control Target Information Related Products

Specifications

Description

GlycoFlux™ MSCV-RBCK1 Viral Particle provisions RBCK1 (HOIL-1) to dissect LUBAC-dependent NF-κB signaling and glycogen-storage phenotypes; MSCV supplies replication-defective, stable genomic insertion in hematopoietic/lymphoid settings to build long-lived mammalian lines.

Product Type

Recombinant MSCV Retrovirus

Target

RBCK1

Species

Human

Promotor

MSCV 5' LTR promoter

Packaging System

Retrovirus

Packaging Cell

HEK293T

Application

This MSCV vector is a common retroviral vector system used to achieve high-efficiency, stable gene delivery into target cells, such as hematopoietic stem cells. It ensures long-term, sustained expression by stably integrating the gene into the host cell's genome.

Shipping

Dispatched on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to preserve titer and stability.

Handling Notes

Store at -80°C and avoid repeated freeze-thaw cycles to preserve viral titer. For safety, handle materials in a biological safety cabinet while wearing appropriate PPE (lab coat, gloves, and eye protection).

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Quality Control

Titer Assay

qPCR

Mycoplasma Test

Negative

Sterility

Sterility testing confirmed no microbial contamination.

Transduction Evaluation

Creative Biolabs offers, upon request, in vitro and in vivo transduction assays to verify lentiviral targeting and to quantify transgene expression and functional output.

Insert Identity Confirmation

All Creative Biolabs retroviral vectors undergo PCR-based verification of proviral integration. Cells are transduced across a dilution series of the retroviral preparation; after several days, genomic DNA is isolated, and a defined region of the expected insert is PCR-amplified to confirm correct identity and integration.

Target Information

Full Name

Ranbp2-Type And C3Hc4-Type Zinc Finger Containing 1

Alternative Name

XAP3; XAP4; HOIL1; PBMEI; PGBM1; RBCK2; RNF54; HOIL-1; ZRANB4; C20orf18; UBCE7IP3

Location

20p13

Gene ID

10616

Summary

The encoded protein has functions in protein sequestration, transcription coactivation, and ubiquitin binding. It participates in NF-kappaB signaling and ubiquitination within the LUBAC complex and is linked to glycogen storage disorders.

GlycoFlux™ AAV1-Human RBCK1 Viral Particle (CAT#: GLJF-1025-JF1020)
GlycoFlux™ MMLV-Human RBCK1 Viral Particle (CAT#: GLJF-1025-JF506)
GlycoFlux™ Lenti-Human RBCK1 Viral Particle (CAT#: GLJF-1025-JF249)

GlycoFlux™ MSCV-Human RBCK1 Viral Particle