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GlycoSens™ Glutamine (Gln) Assay Kit, Colorimetry

CAT#: GLJF-0926-HX191

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Specifications
Product Description
The GlycoSens™ Glutamine (Gln) Assay Kit offers an advanced quantitative methodology for evaluating glutamine concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.
Product Type
Oxidation and Antioxidant Series
Target
GLN
Kit Components
Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer
Sample Types
Tissue homogenates, cell lysates, biological fluids
Sample Volume
10 μL - 50 μL
Detection Principle
The system measures chromatic product formation derived from target glutamine activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of Gln levels.
Detection Time
30-60 minutes
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Glutamine
Alternate Names
Glutamine; GLN
Application Notes
Background
As a pivotal biomolecule, glutamine (Gln) plays a central role in complex physiological networks. This constituent influences multiple downstream effects, anchoring the stability of interrelated metabolic chains. Investigating its functional state is crucial for identifying novel biomarkers and therapeutic targets.
Application
Ideal for investigating glutamine (Gln) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.
Key Features & Advantages
Superior Accuracy; Low Background Noise; Highly Stable Signal Output
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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