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Epimerase Development & Production Services

Overview of Epimerase

Epimerases, a fascinating subclass of isomerase enzymes and Glycosyl Functional Enzymes, function as molecular "switch-hitters" capable of reversibly interconverting stereoisomers by altering the configuration around specific asymmetric carbons. These enzymes are ubiquitous in biological systems and play pivotal roles in essential metabolic pathways, including carbohydrate and amino acid metabolism, where they precisely refine molecular structures to meet cellular demands. As a crucial class of Glycan Tool Enzymes, their ability to modulate stereochemistry renders them indispensable for maintaining biochemical equilibrium and enabling diverse biological functions.

Fig. 1. Summary of engineering outcomes for diverse DAEase variants. (OA Literature)Fig.1 Outcomes of the various DAEase engineering studies.1

Service Portfolio

At Creative Biolabs, our custom epimerase production solutions are tailored to meet your specific needs by leveraging a diverse portfolio of enzyme targets. Below, discover the key enzymes driving our customized solutions.

Cellobiose 2-Epimerase Development Services

We offer specialized enzyme development services for cellobiose 2-Epimerase, enabling efficient conversion of substrates in industrial processes.

Cellobiose Epimerase Development Services

Our enzyme development services for cellobiose epimerase ensure high-purity and active enzyme preparations for diverse applications.

D-Psicose 3-Epimerase Development Services

Leverage our enzyme development services for D-Psicose 3-Epimerase to produce rare sugars with precision and scalability.

D-Ribulose-5-Phosphate 3-Epimerase Development Services

We provide tailored enzyme development services for D-Ribulose-5-Phosphate 3-Epimerase, optimizing its performance for biochemical pathways.

D-Tagatose 3-Epimerase Development Services

Our enzyme development services for D-Tagatose 3-Epimerase facilitate the synthesis of sweet-tasting sugars with health benefits.

Galactose Mutarotase Development Services

With our enzyme development services, Galactose Mutarotase is produced with high activity and stability for various analytical and industrial uses.

Glucose-6-Phosphate 1-Epimerase Development Services

We specialize in enzyme development services for Glucose-6-Phosphate 1-Epimerase, supporting metabolic engineering and biocatalysis.

Glucuronate C5-Epimerase Development Services

Our enzyme development services for Glucuronate C5-Epimerase ensure efficient interconversion of sugar acids for biotechnological applications.

Maltose Epimerase Development Services

We offer comprehensive enzyme development services for Maltose Epimerase, enabling the modification of maltose for improved functionality.

Mannose 2-Epimerase Development Services

Our enzyme development services for Mannose 2-Epimerase provide a reliable source of this enzyme for carbohydrate research and development.

Mannuronate C5-Epimerase Development Services

With our enzyme development services, Mannuronate C5-Epimerase is produced with high yields and purity for alginate biosynthesis studies.

N-Acetyl-D-Glucosamine 2-Epimerase Development Services

We provide expert enzyme development services for N-Acetyl-D-Glucosamine 2-Epimerase, supporting the synthesis of chitin-derived compounds.

Tagaturonate-Fructuronate Epimerase Development Services

Our enzyme development services for Tagaturonate-Fructuronate Epimerase facilitate the interconversion of uronic acids for carbohydrate metabolism research.

UDP-Glucuronic Acid 4-Epimerase Development Services

We specialize in enzyme development services for UDP-Glucuronic Acid 4-Epimerase, crucial for glycosaminoglycan biosynthesis and drug development.

Learn How We Can Accelerate Your Research - Request a Consultation Today.

Epimerases Development & Production Workflow

Step 1

Gene Selection & Vector Prep

Pick a highly active, regioselective epimerase gene essential for the target reaction. Clone this gene into a well-engineered microbial vector. The vector is tailored to ensure efficient replication and high-level expression of the gene in host cells like bacteria or yeast, enabling mass production of pure, active enzyme.

Step 2

Substrate Pre-treatment

Prepare high-purity starting substrates. Solubilize them under conditions that stabilize the epimerase and prevent non- specific reactions. Optimize the choice of co-substrates and reaction buffer through experiments to maximize the enzyme's efficiency for a smooth and selective reaction.

Step 3

Host Cell Transformation

Make host cells competent for DNA uptake, usually via chemical treatment. Insert the vector with the epimerase gene into the cells using heat-shock or electroporation. Plate the cells on antibiotic-containing medium. Only cells that take up the vector will grow into colonies, allowing us to select transformed cells.

Step 4

Expression & Bioreactor Cultivation

Culture the transformed host cells in a suitable medium. Monitor cell density and add an inducer at the right growth stage to start gene transcription. Transfer the culture to a bioreactor and fine-tune reaction conditions like temperature, pH, aeration, and agitation. These optimized conditions boost enzyme production and ensure proper folding and activity.

Step 5

Enzymatic Reaction in Bioreactor

Add the purified epimerase enzyme, pre-treated substrates, and co-substrates to the bioreactor. Continuously monitor and adjust reaction conditions in the bioreactor to maximize the conversion of starting substrates into desired products. This may involve controlling temperature, pH, and nutrient/gas flow rates.

Step 6

Product Separation & Purification

The significant property changes between starting substrates and products make separation easy. First, use sequential liquid-liquid extraction to separate products based on solubility. Then, apply advanced preparative chromatography, such as ion-exchange, size-exclusion, or affinity chromatography for high-purity purification.

Step 7

Epimerase Quality Check

Run SDS-PAGE to assess enzyme purity. A pure sample shows a single band at the expected molecular weight. Test enzyme activity with specific substrates under optimized conditions and quantify products using HPLC or spectrophotometry.

Fig. 2. Epimerase development workflow. (Creative Biolabs AI)Fig.2 Production workflow of epimerases.

Service Highlights

Diverse Expression Platforms

Diverse Expression Platforms
We employ a broad range of expression platforms, encompassing insect cells, mammalian cells, E. coli, and yeast. Each system has distinct merits, allowing us to tailor the choice to your enzyme's needs, ensuring top-notch expression and proper functionality.
Flexible Production Scales

Flexible Production Scales
Our production scales are highly adaptable, spanning from μg to mg levels. Whether for small-scale pilot runs or large-scale commercial production, our system can perfectly meet your demands, optimizing time and resource utilization.
Rich Assay Platforms

Rich Assay Platforms
We utilize multiple advanced assay platforms. These include luminescence for high-sensitivity enzyme activity detection, UV absorbance for straightforward substance quantification, fluorescence for precise measurements, and immunoassay for antibody-antigen-based enzyme detection.
Vast Development Experience

Vast Development Experience
Having developed hundreds of active enzymes over the years, our team boasts extensive assay development experience. From custom assay design to existing assay optimization, our expertise enables us to proficiently handle enzyme-related challenges and deliver high-quality outcomes.

Working with Us to Promote Your Success!

Applications

  • Functional Sugar Production

Epimerases are the linchpin in the synthesis of rare sugars like D-allulose and tagatose from readily available substrates such as fructose and galactose. These rare sugars, with their low-calorie content and potential health benefits, are revolutionizing the food and beverage industry as healthier alternatives to traditional sweeteners.

  • Pharmaceutical Synthesis

In the pharmaceutical realm, epimerases offer stereoselective and environmentally friendly routes to synthesize chiral intermediates and active pharmaceutical ingredients (APIs). Their ability to perfectly control molecular stereochemistry is invaluable in the production of complex drugs with specific pharmacological properties.

  • Biorefinery and Biofuel Production

Epimerases play a vital role in the valorization of lignocellulosic biomass in biorefineries. By converting specific sugars into more readily fermentable forms, they enhance biofuel yields and contribute to the development of a sustainable bioeconomy.

FAQs

Q: How long does the epimerase development process typically take, and what factors influence the timeline?

A: The timeline varies depending on the complexity of the project, including factors such as the availability of starting materials, the extent of enzyme engineering required, and the scale of production.

Q: Can you develop epimerases for non-standard or novel substrates that haven't been studied before?

A: Yes! Our enzyme discovery and engineering capabilities are second to none, enabling us to tackle a wide range of substrates, including novel and complex molecules. We thrive on challenges and are excited to discover new frontiers in epimerase-catalyzed reactions.

Q: Can you modify the epimerase gene to improve its catalytic activity?

A: Our team has extensive experience in genetic engineering techniques. We can use site-directed mutagenesis to introduce specific amino acid substitutions in the epimerase gene, aiming to optimize the active site structure, enhance substrate binding, or improve the catalytic mechanism.

Q: How do you ensure that the genetically modified epimerase is safe for use?

A: Safety is our top priority. Before introducing any genetic modifications, we conduct a thorough risk assessment to evaluate the potential impact on the enzyme's function, stability, and toxicity.

Customer Reviews

Experienced Team
"Working with this team was a game-changer for our rare sugar production project. Their expertise in epimerase engineering and production allowed us to achieve higher yields and product purity, giving us a competitive edge in the market. Their professionalism, responsiveness, and commitment to excellence were truly impressive."- Dr. Jami*** Zaki.

Boosted Production Efficiency
"Partnering with this team transformed our biochemical projects. Their epimerase expertise boosted production efficiency, enhancing both yield and quality, giving us a market lead. Impressed by their professionalism, quick responses, and relentless pursuit of excellence."- Prof. Alex*** R. Thompson.

How to Reach Us

Our epimerase development and production service offers an end-to-end solution for harnessing the power of biocatalysis with precision and innovation. Let's embark on this exciting journey together and unlock the full potential of epimerases! Contact us now!

Reference

  1. Tan, Jin Hao, et al. "The engineering, expression, and immobilization of epimerases for D-allulose production." International Journal of Molecular Sciences 24.16 (2023): 12703. Distributed under an Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/ijms241612703.
For Research Use Only.

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