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Quantitative Human 5HTR2C (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX347

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Specifications
Product Description
The quantitative human 5HTR2C (glycosylated) sandwich ELISA kit is designed to detect human 5-hydroxytryptamine receptor 2C (5HTR2C) levels. 5HTR2C is a G protein-coupled receptor, which binds to serotonin. 5HTR2C plays a role in regulating appetite. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.095 ng/mL, which can accurately detect low concentrations of 5HTR2C in the sample.
Target
5HTR2C
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.095 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
5-Hydroxytryptamine receptor 2C
Alternate Names
5HTR2C; 5-Hydroxytryptamine receptor 2C
Uniprot No.
N-Glycosylation Site
39, 204
Application Notes
Application
The quantitative human 5HTR2C (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated 5HTR2C levels. It is useful for studying the role of 5HTR2C in food intake.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-105%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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